The second edition of the international temporal bone dissection course on cadavers took place from September 25 to 26, 2024, at the premises of the Department of Anatomy at the 2nd Faculty of Medicine, Charles University. The course focused on training surgical techniques for the treatment of acute and chronic otitis and the surgical treatment of deafness. It was primarily intended for ENT (otolaryngology) residents, who had the opportunity to acquire skills under the guidance of experienced experts. The event was highly successful, and another edition of the course is planned for two years from now.